A simple, rapid, accurate, precise and reproducible RP-HPLC method was developed and validated for the estimation of guaifenesin in tablet dosage form. The method was carried out using Enable C18 G 250 x 4.6 mm, 5 µ column in a binary mode with mobile phase water adjusted to pH 3 with OPA and acetonitrile in the ration of 73:27% v/v. Flow rate was set at 1.3 ml/min and detection was carried out at 240 nm using a PDA detector. The retention time was found to be 5.281min and the method produced linear response in the concentration range of 2-20 µg/ml (r2-0.999). The recovery studies were also carried out and % RSD from reproducibility was found to be below 2%. LOD and LOQ for this method were found to be 0.104 µg/ml and 0.318 µg/ml respectively. The developed method was simple, sensitive and specific. Hence the method can be used for the estimation of guaifenesin in tablet dosage form.
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